Background: Since in the process of Assisted Reproductive Techniques (ART) such as (Intra Cytoplasmic Sperm Injection) ICSI and IVF (Invitro Fertilization), human intervention and laboratory conditions may affect fetal development, whatever the used method is aggressive, disorders are more likely to occur in children. In addition to the progress and the promotion of assisted reproductive therapies, physical, behavioral, speech and language development of the children born through these methods is of particular importance. The current study aimed to compare the spoken language skill in a pair of IVF twins, a pair of fraternal twins and two singleton children.Methods: The participants of the study were selected randomly from the kindergartens of Tehran City. All of the children were Farsi-speaking females and they were four years old. In this study, the valid and reliable Test of Language Development (TOLD) was used. For studying the spoken language, skills of sentence combining, picture vocabulary, word ordering, generals, grammatic comprehension, and malapropisms were analyzed. Findings: The results of the study showed that no significant differences among scores of the composite quotients of three groups including IVF twins, fraternal twins and two singleton children in the compound subtest of spoken language were found and IVF infants scored slightly lower than control children. Obtained scores for the three groups were (110 and 102), (121 and 108) and (116 and 120) respectively.Conclusion: It seems that IVF children are not at great risk of speech and language development delay.